sabry.mahmoud

sabry.mahmoud

استاذ - أستاذ ورئيس قسم الميكروبيولوجى

كلية الزراعة

العنوان: 105 شارع الهلالى - شقه 215- برج عمر بن الخطاب - أسيوط - مصر

5

إعجاب
Isolation and biologically purification of red clover mottle virus: First record on broad bean in Egypt
Red clover mottle virus was isolated and biologically purified by single local lesion method on Phaseolus vulgaris plants. The obtained isolate was mechanically transmitted and has narrow host range included most plants belonging to Fabaceae, Solanaceae and other weed plants. Red clover mottle virus (RCMV) doesn't transmit through Myzus persicae and Aphis craccifora insects. Electron microscopy (EM) has revealed presence ... إقراء المزيد

Detection and partial characterization of a putative closterovirus affecting Ficus carica: molecular, ultrastructural and physiological aspects of infected leaves
_Fig mosaic disease_ (FMD) was systemically transmitted to susceptible fig plants by grafting and led to development of symptoms including a wide array of discoloration, green blisters, mottling, crinkling and deformation of leaves and fruits. A sensitive assay for the detection of FMV using specific enzyme-linked immune sorbent assay and reverse transcription-polymerase chain reaction was developed. A double-stranded RNA ca. ... إقراء المزيد

2011 | الكلمات المفتاحية DAS-ELISA, CMV, dot blot, Western blot, bioassay, nonspecific reactions,
Detection of cucumber mosaic virus in some ornamental plants and elimination of nonspecific ELISA reactions
During surveys carried out in 2008 in the nurseries of some ornamental and medical plants, about 90 plant samples belonging to six plant species were collected. Cucumber mosaic virus (CMV) was detected by routinely double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) in most tested plants. In _Vinca rosea, Ocimum basilicum_ and _Pelargonium_ sp., which reacted positively for CMV, 100% of ... إقراء المزيد

2011 | الكلمات المفتاحية BBSV, identification, Electron microscopy, broad bean, nodulation,
Identification and detectability of broad bean stain virus in broad bean seeds and effects on nodulation
During a survey for broad bean viruses in Sohag Governorate, a virus was identified as Broad bean stain virus (BBSV), a member of the genus Comovirus, based on host reactions, symptomatology, electron microscopy and serologically by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) technique. The virus was mechanically transmitted to some members of Leguminosae and also through seeds. The ultraviolet ... إقراء المزيد

2010 | الكلمات المفتاحية
Antiviral Activity of latex from Ficus nitida against plant viruses
Latices tapped from Ficus elastica, F. nitida and Euphorbia pulcherrimia plants were screened for antiviral activity against some plant viruses in different hosts. Three models of host-virus interaction were used. Two systemic infections, squash-zucchini yellow mosaic virus (ZYMV); broad bean-bean yellow mosaic virus (BYMV) and the third one, bean-tobacco necrosis virus (TNV) as local infection. The greatest degree of antiviral ... إقراء المزيد

2010 | الكلمات المفتاحية
Tobacco callus culture as a propagating medium for cucumber mosaic Cucumovirus.
This study was performed on Cucumber mosaic _cucumovirus_ (CMV) as a model. The virus was isolated from naturally infected cucumber plants depending on polyclonal antibodies specific for the CMV (subgroup I) and local lesions produced on _Chenopodium amaranticolor_ were used as a source of single lesion isolation. CMV isolate was maintained in _Nicotiana tabacum_ cv. White Burley. The presence of ... إقراء المزيد

Modifications of antioxidant activity and protein composition of bean leaf due to Bean yellow mosaic virus infection and salicylic acid treatments
The antioxidant status as well as protein composition of faba bean leaves infected with _Bean yellow mosaic virus_ (BYMV) and the effect of salicylic acid application was examined in this paper. Some modifications in the antioxidant status were observed by changing some antioxidant enzymes activities and contents of antioxidant metabolites. BYMV-infected leaves revealed POD, CAT, APX and SOD induced activities ... إقراء المزيد

2010 | الكلمات المفتاحية AMV, DAS-ELISA, RCMV, RT-PCR, neclotide, necliotide secuance, necliotide sequances,
Differentiation Study Between Alfalfa Mosaic Virus and Red Clover Mottle Virus Affecting Broad Bean by Biological and Molecular Characterization
This study aimed to identifying the causal virus (es) inducing wilting and necrotic symptoms on broad bean plants. Amplification of total RNA extracted from infected broad bean yielded 200-550 bp using Comovirus-specific primers. The amplified cDNAs was cloned, sequenced and analyzed. Nucleic acid sequence analysis of smallest and biggest bands revealed of 100 and 95% sequence identity with other Red ... إقراء المزيد

2009 | الكلمات المفتاحية
Evaluation of some therapies to eliminate potato Y potyvirus from potato plants
Viral infection of potato (_Solanum tuberosum_ L.) plants is very important due to their effect on potato yield and degeneration of seed tubers. This study aimed to eliminate Potato Virus Y (PVY) by tissue culture technique using different therapies, such as thermo-, chemo- and electrotherapies. Potato plants cv. Diamond cultivated at Faculty of Agriculture farm, Sohag University were tested by ... إقراء المزيد

Coat protein-mediated resistance as an approach for controlling an Egyptian isolate of Cucumber mosaic virus (subgroup I)
Cucumber mosaic virus (CMV, cucumovirus) is the most important virus infecting cucurbit crops in Egypt and worldwide causing significant loss in yield quality and quantity. The main target of the present work was to establish a simple controlling system for an Egyptian isolate of such virus (belonging to the subgroup I) via production of tobacco transgenic plants expressing viral coat ... إقراء المزيد