The biological effect of the perfluorocarbon-based artificial oxygen carrier (OxygentÔ) was investigated in tissue- engineered trachea (TET) construction. Media supplemented with and without 10% Oxygent were compared in all assessments. Partial tissue oxygen tension (PtO2) was measured with polarographic microprobes; epithelial me- tabolism was monitored by microdialysis inside the TET epithelium perfused with the medium underneath. Chondrocyte–DegraPolÒ constructs were cultured ... Read more

Optimum graft function after liver transplantation is dependent on adequate sinusoidal perfusion. Hepatic microcirculation may be compromised by sev- eral factors such as cold ischemia, quality of the liver graft, handling of the organ during surgery, surgical procedure, and reperfusion injury. This chapter will focus on the pathological consequences of cold preservation and reperfu- sion injury on hepatic microcirculation. Microcirculatory ... Read more