This study gives highlights on the biodiversity of spiders in an olive trees orchard, a lemon trees orchard, and webs of Cyrtophora citricola at Jazirat Shandweel District, Sohag Governorate, Egypt. In addition, it gives a preliminary list of spiders based on the main taxonomic characters. The sampling was carried out weekly or monthly during the period: September, 2015 to January, 2018 using the beating net and hand picking methods. A total of 6237 individuals were collected, identified, and classified into: 30 species belonging to 29 genera and 21 families; among them, three families, 18 genera, and 11 species are listed in addition to other unidentified species.