Background and aim of the study: In the last 2 decades, a relation between Helicobacter pylori (HP) chronic infection and migraine has been studied but the results of these studies are controversial. The aim of this study was to determine whether current or previous exposure to HP infection is a risk factor in women with non-menstrual migraine without aura, a concise subgroup in which environmental factors are dominant. Methods: A total of 24 women, with age ranged from 19 - 60 years, affected by non-menstrual migraine without aura were evaluated in comparison with control subjects consisted of 24 women, with age ranged from 20 - 59 years, without any primary headache history. In both cases and controls HP infection was evaluated by detection of HP antibodies in serum and HP antigen in stool. Results: The prevalence of HP infection was insignificantly higher in the migraineurs without aura compared to controls (p=0.24 and p=0.33). Regarding the intensity, duration, and frequency of attacks of migraine no differences were detected between both groups. Conclusion: our findings do not support that HP infection is a risk factor for non-menstrual migraine without aura.