Tumor necrosis factor-α (TNF-α) polymorphism has been
suggested to play an important role in the pathogenesis of non alcoholic fatty
liver disease (NFALD), and known to be a mediator of insulin resistance. Aim of
the work: to investigate the influence of G 308 A (allele TNFA) & G 238 A (allele
TNF2) TNF -α polymorphisms and its relationship with insulin resistance. Patient
and Method: Sixty patient with NAFLD AND 30 controls were tested by
homeostatic metabolic assessment insulin resistance (HOMA-IR),serum level of
Human Insulin and TNF -α assay by ELISA kit and TNF -α polymorphism by
polymerase chain reaction (PCR) analysis Result: Insulin resistance was detected
in all patient and was highly significant in NAFLD in comparison with control (
4.32±1.96 vs 1.75±0.48 ) respectively,there is significant value In TNF -α 238
allels polymorphism in NAFLD in comparison with control ( P=0.048 )but not in
308 gene , and Multivariate regression analysis showed that the only independent
risk factors for development of NAFLD included higher body mass index (BMI)
and higher HOMA-IR index. Conclusions: we conclude that TNF -α
polymorphism could represent a susceptibility genotype for insulin resistance
and NAFLD.