Non-pathogenic protozoa are single-celled parasites usually detected in the intestinal tract and with a
worldwide distribution. Our aim in this study was to estimate the non-pathogenic intestinal parasites among primary schoolchildren in Sohag Governorate, Egypt and to correlate between these protozoa and the release of symptoms. Stool specimens of 200 child were investigated microscopically by iodine stained smear and formol-ether sedimentation. Non-pathogenic protozoa represented 9% of all studied children (3.5% for Entamoeba coli, 2.5% for Iodamoeba bütschlii and 1.5% for each Entamoeba hartmanni and Chilomastix mesnili). Iodamoeba bütschlii and Entamoeba coli infection showed a statistical significance regarding symptoms. Only Iodamoeba bütschlii infection was affected statistically by the child gender. Infection with these non-pathogenic parasites is a proof of fecal contamination and can also be disordered with the potentially pathogenic parasites. In addition, these parasites are also problematic in that they may
be considered the cause of some symptoms after exclusion of other pathogens.