During a survey for broad bean viruses in Sohag Governorate, a virus was identified as Broad bean stain virus (BBSV), a member of the genus Comovirus, based on host reactions, symptomatology, electron microscopy and serologically by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) technique. The virus was mechanically transmitted to some members of Leguminosae and also through seeds. The ultraviolet absorption spectrum of the purified virus showed a typical curve of nucleoprotein with an A260/280 ratio of about 1.25–1.34. The yield of purified virus was 0.61–0.63 mg/kg infected tissue. Electron microscopy of purified preparations revealed the presence of isometric virus particles, 27 nm in diameter. The polyclonal antibodies against BBSV were produced and the antiserum titre of three bleedings was determined by indirect DAS-ELISA technique. Gross reduction of …