This study was carried out at the experimental buffalo farm of the Animal Production Department, Faculty of Agriculture, Minufiya University, Shebin El-Kom, Egypt. In this experiment, behavioural activities of lactating buffalo cows were used in either tie stall or loose housing to evaluate housing systems comfort. Five lactating buffalo cows in their fourth lactation were used with body weight averaged 608 ±14.21 kg in the beginning of the experiment. The behavioural activities of lactating buffalo cows were observed during two periods (each period lasted 168 hours for 7 days). In the first period buffaloes were housed in tie stall barn with hard floor surface, in the second period the same buffaloes were housed in loose housing barn with hard floor surface with total space about 40 m2 (8 m2 per each lactating buffalo). Mean frequency of eating behaviour was significantly higher in loose housing than in tie stall (10.23 ± 0.12 vs. 8.14 ± 0.15 respectively). Furthermore, mean duration of eating behaviour was significantly higher in loose housing than in tie stall (251.09 ± 4.27 vs. 202.26 ± 4.34 min. respectively). Increasing in eating duration and frequency in loose housing may be due to that buffalo cows have greater opportunities to display the normal behavioural changes than in tie stall. Otherwise, there was no significant difference between loose housing and tie stall systems in frequency of ruminating behaviour (12.83 ± 0.19 and 12.31 ± 0.22 respectively). The Ruminating (R):Eating (E) ratio obtained in our study was 2.1 and 1.8 for Buffaloes in tie stall and loose housing systems respectively. Buffaloes in loose housing system spent longer total lying time at the expense of total standing time which was shorter in comparison with those in tie stall (635.03 ± 12.95 and 804.97 ± 12.95 vs. 559.71 ± 16.49 and 880.29 ± 16.49 min. respectively). Lying and standing frequency are significantly higher in loose housing than in tie stall (9.14 ± 0.34 and 10.14 ± 0.34 vs. 6.86 ± 0.14 and 7.86 ± 0.14 respectively). Most commonly observed head position during lying was head up .Fight, butting and head against were significantly higher in loose housing than in tie stall (0.34, 0.68 and 0.73 vs. 0.12, 0.31 and 0.29 placement frequency/day respectively). Buffalo cows housed in loose housing have shown a greater number of agonistic interactions than buffaloes in tie stall (2.69 ± 0.15 vs. 1.57 ±0.12 placement frequency/day respectively). Furthermore some physiological parameters such as body temperatures, respiration rate and pulse rate were also studied.