This study was carried out at the behavioural research Unit of Animal Production Department, Faculty of Agriculture, Minufiya University, Shebin El-Kom, Egypt. In this experiment, behavioural activities of buffalo calves were used in either tie stall or loose housing to evaluate housing systems comfort. Continuous observation methods were used to record the activity patterns of sixteen male buffalo calves during two periods (each period lasted 168 hours for 7 days). In the first period buffalo cows were housed in tie stall barn with hard surfaces, in the second period the same buffalo calves were housed in loose housing barn with hard surfaces with total space about 80 m2 (5 m2 per calf). Mean frequency of eating activity was significantly higher in loose housing than in tie stall (7.89 ± 0.05 vs. 7.14 ± 0.06 respectively). Eating periods were closely coincided with daily feeding times. Furthermore, mean duration of eating behavioural activities was not significantly affected by the housing system (251.22 ± 2.74 vs. 244.91 ± 2.96 min for loose housing and tie stall respectively). On the other hand, no significant difference was found between loose housing and tie stall in frequency of ruminating behavioural activities (13.41 ± 0.13 and 13.27 ± 0.12 respectively). Meanwhile, duration of ruminating behavioural activities was significantly longer in loose housing than tie stall (399.58 ± 3.17 vs. 387.68 ± 3.86 min respectively). It seems that there were two main peaks of ruminating period in either loose housing or in tie stall (12:00 to 15:00 and 21:00 to 24:00h). Calves spent total lying time significantly shorter in loose housing than in tie stall (567.16 ± 3.22 vs. 608.62 ± 2.69 min. respect.). Lying frequency was significantly higher in loose housing than in tie stall (9.03 ± 0.08 vs. 8.29 ± 0.09 times respect.). Duration of resting bouts was significantly higher in loose housing than in tie stall (235.64 ± 11.21 vs. 169.91 ± 10.91 min. respectively). The frequency of resting bouts was significantly lower in loose housing than in tie stall (1.43 ± 0.08 vs. 1.67 ± 0.08 respectively). These findings may reflect the disturbance of buffalo calves in tie stall that forced them to change their resting behaviour. Calves lying in equaled period in daylight and night for both housing systems. Space allowance of 5m2 for each calf in loose housing system resulted in more frequency of standing and lying behavior, with longer standing time and increasing of agonestic behavioural activities. Our results suggest a lack of comfort for calves raised in tie stall system, which may be apparent in reduced time spent for lying and a subsequent increase in time spent standing without eating. This could be attributed to that calves in tie stall had difficulty to standing up and lying down. There was also a tendency for significantly higher physiological parameters studied, such as rectal temperature, respiration rate and pulse rate in loose housing.