This study was carried out at the Experimental Buffalo Unit of the Animal Production Department, Faculty of Agriculture, Minufiya University, Shebin El-Kom, Egypt. Nine dairy buffalo cow at 2nd to 4th lactation with an average body weight 614 ± 24.08 kg were randomly assigned among three experimental tested rations (three animals each). The experimental rations were Control ration (42% commercial concentrate feed mixture + 33% berseem hay + 25% rice straw), Control ration + 3% Tafla/animal/day and Control ration + 20 g Baker’s yeast/animal/day (on dry matter basis). Fresh manure sample was collected from each animal daily and physical, chemical and biological analyze were applied. Manure from animals fed tafla supplemented ration had the lowest (P<0.01) odor intensity followed by manure from animals fed yeast additive ration, while manure from animals fed control ration had the highest odor intensity (1.83±0.058, 2.42±0.048 and 2.68±0.064, respectively). The similar differences were evident for moisture percentage (78.90±0.23, 80.60±0.26 and 84.38±0.26 % respectively). The effect of adding tafla and yeast on manure pH value was highly significant (P<0.01) but within normal range. Manure from animals fed tafla supplemented ration was lower in nitrogen, phosphorus and potassium % (P<0.01) than either manure from animals fed yeast additive ration or manure from animals fed control ration (0.433- 0.105- 0.377, 0.467- 0.108- 0.374 and 0.498- 0.121- 0.395 %, respectively). After 6, 12, 24h of incubation manure from animals fed tafla supplemented ration had the lowest gas production followed by manure from animals fed yeast additive ration, while manure from animals fed control ration had the highest gas production (0.633- 1.485- 2.767, 0.674- 1.570- 3.093 and 0.893- 1.903- 4.341 ml/g manure, respectively). Manure from animals fed tafla supplemented had the lowest (P<0.01) coliforms count followed by manure from animals fed yeast additive, while manure from animals fed control ration had the highest coliforms count (5.31±0.020, 5.44±0.012 and 6.46±0.015 cfu Log10/g manure, respectively).