This experiment was designed to study the effect of animal species, bedding materials and storage periods on environmental pollution of farm animals’ manure. Manure samples of different animal species (buffaloes, Baladi cattle, sheep, goats, camels, horses and donkeys) collected from El-Raheb farm were used and mixed with different bedding materials (sand, straw, sawdust and newspaper) in wide plates and were left at room temperature for 28 days. The physical, chemical and biological analyses were determined at 1st, 2nd, 3rd, 7th, 14th and 28th day. All physical (color, odor, moisture and pH), chemical (N%, P% and K%) and biological characteristics (total coli forms count) were significantly affected by different types of bedding materials. Color was not affected by storage period. On the other hand, odor, moisture and pH were decreased significantly by storage period. Also, the same result was obtained for N%. However, P% and K% were not significantly affected by storage period. Total coli forms count were significantly affected by storage period. Manure had the highest (P<0.01) coli forms count during the first three days of storage then started in reduction from 7th and 14th days to touch bottom at day 28.