Title: CD36  in Acute Myeloid Leukemia in Correlation to Blood Picture and Clinical Variables

Hasnaa A Abo-Elwafa, Mohammed A Mohammed, Ahmed A Allam and   Ahmed M Sayed

Clinical Pathology Department, Sohag Faculty of Medicine, Sohag University, Egypt

Authors affiliations:

1- Hasnaa Ahmed Abo- Elwafa

Corresponding author

Hasnaa_aboalwafa@med.sohag.edu.eg

Assistant Prof. of Clinical Pathology

Sohag Faculty of Medicine, Sohag University, Egypt

2-Mohammed Abdallah   Mohammed

Lecturer of Clinical Pathology

Sohag Faculty of Medicine, Sohag University, Egypt

3- Ahmed Ahmed Allam

Lecturer of Clinical Pathology

Sohag Faculty of Medicine, Sohag University, Egypt

5-Ahmed Mohammed Sayed Ahmed

Assistant Lecturer of Clinical Pathology

Sohag Faculty of Medicine, Sohag University, Egypt

Ethical considerations: This work has been approved by ethical committee of Sohag Faculty of medicine, according the ethical considerations l standards of the institutional and national research committee and with the Helsinki 1964 declaration. Written informed consent was obtained from all women participate in the research; they informed that the blood samples for research purpose. 

Conflict of Interest:  all authors declare that they have no conflict of interest.

Funding: The research funded by Sohag University Hospital. 

 

 

 

CD36 Expression in Acute Myeloid Leukemia in Correlation to Hematological  and Clinical Variables

By

Hasnaa A Abo-Elwafa, Mohammed A Mohammed, Ahmed A Allam and   Ahmed M Sayed

Clinical Pathology Department, Sohag Faculty of Medicine, Sohag University, Egypt

Abstract, background, CD 36 is an integral membrane protein, member of the class B scavenger, receptor involved in phagocytosis of neutrophils and T-lymphocytes, aims to investigate  expression of CD36 in acute myeloid leukemia specially monocytic lineage involved leukemia  (MLIL) cells  exploring its association with  disease severity, subjects and methods, this  study was carried on (30) newly diagnosed  acute myeloid leukemia (AML) patients, aged from 17 to 70 years , and 20 healthy subjects of the same  age as a control, on the period from October 2013 to October 2014, revised by the Scientific Ethical Committee of Sohag University Hospital , written informed consent was taken from all participants, complete blood count  using Cell-Dyne 3700, ABBOTT diagnostic (USA), peripheral blood (P. Bl.) and bone marrow (BM) examination, immunophenotyping (IPT) using Becton Dickinson FACS Canto Caliber four color flow cytometer (USA),for CD13,CD33,MPO, CD117 ,CD14,CD15 ,CD65,CD68  and determination of blast cells percentage expressing CD36, Mo Abs supplied by B.D Bioscience, USA, Cat. No. 656151, results, there were significant positive correlation between CD 36 expression and percent of BM blasts also organomegaly and lymphadenopathy (r = 0.50; p = 0.04), there was positive significant correlation between CD36 expression and CD13, D14, CD15 (r = 0.62, 0.0; p = 0.05),positive rate of CD36 expression in AML(46.7%), and in MLIL (100%),while CD14 expression positive rate in MLIL (64.3%)and CD15 (71.4%),conclusion, the combination of CD36 with myeloid antigens is helpful to differential diagnosis of  MLIL, positive rate of CD36 is higher than that of CD14 and CD15 in MLIL.                     

 Key words: CD36, AML, MLIL.