Comparison between second generation of functional cybrid (containing Solanum nigrum genome and Solanum tuberosum plastome) and original (S. nigrum) plants was used to study the effect of nucleus/chloroplast interaction on the physiology of the respective cybrid. Percentage of seed germination, response of shoot apices to form adventitious shoots on B5 medium supplemented with 5% liquid coconut endosperm and 2.5 M BAP, and morphological characters of cybrid were similar to that of the original plant. The cybrid shoots were light green and bleached under atrazine stress while the original plant shoots were dark green and were not affected by atrazine. Fresh and dry matter as well as carbohydrate contents of cybrid shoots were lower than those of original plant, generally, these values were reduced under atrazine stress. Protein and sodium contents remained unaffected under chloroplast substitution and atrazine stress. Potassium content of cybrid was higher than those of corresponding original plant, but with a tendency to be lowered under atrazine stress. X-ray microanalysis data showed differences in ion contents between the cybrid and original plant on atrazine free medium or under atrazine stress.