Testone – E (TE) is an anabolic androgenic steroids (AAS) and widely used as a medical drug which possesses multiple clinical therapeutic benefits. The aim of this study was to determine the effects of TE on the ovarian tissue of adult female mice and the possible protective role of ascorbic acid (AA). Materials and Methods: Thirty adolescent (21- to 28-day-old) female Swiss albino mice (17-20g) were equally divided into 3 groups. The first group (GI) served as control. The second group (GII), was injected intraperitoneally (IP) with TE at a dose (25mg/kg) daily for four weeks. The third group (GIII) was injected (IP) by TE (25mg/kg) concurrently with AA at a dose (100mg/kg) daily for four weeks. Results: Remarkable histopathological changes occurred in the ovaries of TE-treated group, including degenerative changes in the ovary with many atretic follicles and vacuolated stroma. Ultrastructural examination showed the follicles in TE-treated group appeared to have vacuolated oocyte with nuclear shrinkage and the granulosa cells demonstrated some signs of degeneration. Also, estrogen (ER) and progesteron (PR) showed weak immunoreactivity within the cells in TE-treated group. The above mentioned toxic effects in TE-treated group were markedly improved by treatment with AA. Conclusion: TE caused detrimental effects on the ovarian tissue; However, AA prevents the harmful effects of TE on the ovarian follicles.