The study was carried out on 40 patients with Alopecia Areata (AA) attending Dermatology Outpatient Clinic of Sohag University Hospital. 85% had localized AA and 15% had alopecia totalis. 10 healthy individuals were selected as a control.The age of patients ranged from 6 to 50 years with a mean 23.93±12.42 years. Males were 62.5% and females were 37 .5 % .The duration of the disease ranged from 2 days to 4 years with a mean (155.40±303.35). Patients had AA less than one month were 42.5%, while from one month to 1 year were 47.5% and more than one year were 10%. The course of the disease was stationary in 30% and was progressive in 70%. All patients and control group were subjected to the measurement of serum IL-1β, IL-4, INF-γ (by Medgenix EASJATM kit) and TNF-α. (by Immulite Chemiluminescent Enzyme ImmunometricAssay kit). The serum level of IL-4 was significantly decreased in all patients in comparison with control. Also it was found that the concentration of IL-4 decreased by prolongation of the disease duration.The serum levels of IL-1β, INF- γ and TNF- α. showed no significant difference. Further studies are recommended for comparing the cytokines profile in both localized AA patients and totalis/ universalis group of patients.