Background: Acrylamide (ACR) is a water soluble vinyl monomer that has multiple chemical and industrial applications. Several studies were used to define its side effect on liver, kidney, brain and erythrocyte which proved that (ACR) increased glutathione-s-transferase and causing oxidative stress. Ascorbic acid is natural antioxidant that prevents the increased production of free radicals induced by oxidative damage to various cellular components. Theaim of work : This work aimed to the study the effect of ACR on the jejunum of adult male albino rats and to evaluate the possible protective role of ascorbic acid. Material and methods: thirty albino rats (2-3 months old) were used in the present study. Their weights ranged from 200-250 grams. The rats were divided into 5 group; Group Ι: were used as negative control group .Group IIa: received ACR ,30 ml\kg orally daily for 4weeks .Group IIb : received both ACR, 30 ml\kg orally simultaneously with ascorbic acid 400ml \kg. intraperitoneally daily for 4weeks. Group IIIa : received ACR 80 ml\kg orally for 4weeks.Group IIIb :received both ACR 80 ml\kg orally simultaneously with ascorbic acid 400ml \kg. intraperitoneally daily for 4weeks.The jejunum were dissected and processed for histological and immunohistochemical studies . Results: Dose dependent histopathological changes of The jujenal villi and muscuolosa were observed after ACR treatment . There were marked inflammatory cell infiltrations and vascular congestion in the lamina propria, submucosa and musculosa. There was increase in the PCNA and AFP positive cells . All these changes could be significantly attenuated by using ascorbic acid with ACR. Conclusion: Fried food should not be used so frequent and if no way to be used , it is essential to use meals containing ascorbic acid .