Five Cu(II) complexes are tridentate Schiff bases amino acids ligands derived from condensation 3–methoxysalicylaldehyde (MS) or 4-diethylaminosalicylaldehyde (DS) with α-amino acids (L-phenylalanine(P), L-histidine (H), DL-tryptophane (T)). All ligands are analyzed by 1HNMR and 13CNMR. The investigated complexes have been prepared and characterized by elemental analysis, infrared spectra, ultraviolet-visible and thermal analysis in dynamic air atmosphere (40ml / min) at a heating rate (10o C / min) from ambient temperature to 750o C, the experimental results indicated that the investigated complexes contain hydrated water expect DSPCu don̓t contain hydrated water. The molar conductance values of Cu (II) complexes were relatively low, indicating the nonelectrolytic nature of these complexes. Magnetic susceptibility measurements show that the complexes are paramagnetic. A good agreement was found between the calculated values of C, H, N and found values in elemental analysis that show the purity of the complexes. Moreover, the stability constants of the prepared complexes have been determined spectrophotometrically. The complexes have been found to posses 1 : 2 ( M : L) stoichiometry. The results suggest that 3-methoxysalicylaldehyde or 4-diethylaminosalicylaldehyde amino acid Schiff bases behave as dibasic tridentate ONO ligands and coordinate to Cu(II) in octahedral geometry according to the general formula [Cu(HL)2].nH2O. The ligands and their complexes show antimicrobial effect against many types of bacteria such as Bacillus subtilis (+), Escherichia coli (-),Micrococcus luteus (+) and many types of fungi such as Asperagillus niger, Candida glabrata, Saccharomyces cerevisiae. The results of these studies indicate that the metal complexes exhibit a stronger antibacterial, antifungal efficiency than their corresponding ligands. Catalytic activities of all complexes have been studies for the oxidation of alcohols using H2O2 as oxidant. Different reaction parameters (such as time of the reaction, temperature and amount of catalyst) were investigated in this reaction and the results indicate that the metal complexes exhibit low to medium activity in oxidation of alcohols. In this group of copper (II) complexes, increasing temperature leads to increasing in reactivity but decreasing selectivity, decreasing time of reaction leads to increasing in selectivity and reactivity and increasing amount of catalyst leads to increasing in reactivity and selectivity. The experimental results indicated that the investigated complexes showed a different selectivity and reactivity of oxidation of alcohols such as benzylalcohol.