Air and moisture stable coordination compounds of first transition metals such as Co(II), Ni(II) and Cu(II) with newly designed Schiff base (bsiph) incorporating 5-bromosalicyaldehyde with isatin and 4-nitro-o-phenylene diamine have been synthesized to elucidate the structure of metal complexes. They are characterized by elemental analyses, Infrared, 1HNMR, 13CNMR, thermal analysis, UV-visible, Conductivity and magnetic susceptibility measurements. The results suggest that the prepared Schiff base ligand behave as mono basic tetra-dentate ONNO ligand and bind to Cu(II), Co(II) and Ni(II) in octahedral geometry. The DNA interaction of these complexes was tested by using electronic spectra and viscosity measurements. DNA cleavage activity of the prepared Schiff base complexes were monitored by gel electrophoresis. Moreover, the prepared compounds are tested for their in vitro antibacterial activity against three types of bacteria, (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) and fungi (Aspergillus flavus, Candida albicans and Trichophyton rubrum). The results show that the metal complexes are more reactive with respect to its ligand. Furthermore, the catalytic activity of complexes towards the oxidation of benzyl alcohol using hydrogen peroxide as oxidant was investigated using gas chromatography .