New Cr (III), Fe (III) and Cu (II) complexes with MSBB (2‐(1‐butyl‐4,5 diphenyl‐1H‐imidazol‐2‐yl)(4-bromophenol) were synthesized. The  multi-substituted aryl imidazole ligand (MSBB) and its complexes were characterized by using elemental and thermal analysis, molar conductivity, magnetic susceptibility, UV-Vis spectral data, IR, and NMR. The obtained data showed that prepared  complexes were 1 :1 (metal : ligand) ratio, octahedral (Oh) for all complexes geometry and nonelectrolytes. Moreover, the investigated complexes were tested for their antibacterial and fungal activity and showed good results of inhibition against the selected pathogenic microorganism. Moreover, the interaction of investigated complexes with CT-DNA was studied by different methods.  Furthermore, the growth inhibitory effect of the investigated compounds was examined on some cancer cells lines.