In order to study salt tolerance of barley (Hordeum vulgare L.) genotypes in germination and field growth stages, laboratory and field experiments were carried out at the Agricultural Experimental Research Farm of Faculty of Agriculture, Sohag University, in 2005/2006, 2006/2007 and 2007/2008 seasons. This work aims to screen and evaluate barley genotypes under salinity conditions. Two hundred and eighty genotypes of barley were screened by germination under salinity with different levels of NaCl in the laboratory. The best twenty selected genotypes under the highest level of salinity of NaCl and the two check cultivars Giza 127 and Giza 128 in laboratory test were evaluated in the next seasons under salinity affected soil in a field experiment. The results indicated that seven out of the twenty genotypes were tolerant to salinity stress. These genotypes were No. 1, 5, 7, 10, 13, 17 and 19. These seven genotypes had salinity susceptibility index (SSI) less than unity. The results revealed that the average of grain yield (ardab/fed) for the highest genotype No.10 was 9.44 ardab/fed, which was significantly higher than that the lowest genotype ( No. 1) by 1.39 ardab/fed. It was also significantly higher than the check cultivars G.127 and G.128 (No. 21 and 22) by 1.04 and 1.59 ardab/fed, respectively. The decrease percentages of grain yield (ardab/fed) for the highest genotype No. 10, the lowest one, No.1 and the two check varieties ( No. 21 and 22) due to salinity increase, were 22.11, 21.71, 18.57 and 24.08% , respectively. Salinity susceptibility index (SSI) for grain yield (ardab/fed) ranged from 0.51 for genotype No.7 to 0.99 for genotype No. 5.