Mahmoudibrahim

Mahmoud Ibrahim Yousef Elbadry

Lecturer - Lecturer of Internal Medicine (Hematology oncology)

Faculty of medicine

Address: Internal Medicine department, Sohag Faculty of Medicine, Sohag University

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Date and place of birth: 15 December 1983, Alexandria, Egypt.
Address: Department of Internal Medicine,Sohag Faculty of Medicine, Sohag University, Sohag, Egypt.
Languages: Arabic, English.

Languages: Arabic, English.


Position: 

1-Lecturer of Internal Medicine, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt.                     

2- Fellow researcher , Cellular Transplantation Biology (Hematology/Respirology) Faculty of                          Medicine, Institute of Medical Pharmaceutical and Health Sciences, Kanazawa  University,Ishikawa Japan.

3-Clinical training:Hematology Departement, Faculty of Medicine, Institute of Medical            Pharmaceutical and Health Sciences, Kanazawa University, Japan. from February 2016 to February 2018
4-Animal experiment training:Animal experiment basic training,Institute of Medical            Pharmaceutical and Health Sciences, Kanazawa University, Japan. July,2017.

5-Radioactive isotopes trainin.Radioactive chromium (Cr) release assay training using a gamma counterInstitute of Medical Pharmaceutical and Health Sciences, Kanazawa University, Japan. Augaust
2016.

6-Society Memberships:Egyptian Stem Cell Transplantation Hematologic Disease Association
April 2015 till now

7-Assistant lecturer:Sohag Faculty of Medicine form 3/2/2013 to 30/11/2019.
8-Demonstrator:Sohag Faculty of Medicine form 1/3/2012 to 3/2/2013.
9-Internal Medicine Resident:Sohag University Hospital, from 1/3/2009 to 28/ 2/2012.
10- House officer “Rotating internship”:Sohag Faculty of Medicine, Sohag University Hospital,Egypt. from 1/3/2008 to 28/2/2009.


(II)QUALIFICATIONS:

Medical doctorate:October 2019October 2019

Medical doctorate study:*Kanazawa University, Faculty of Medicine, Institute of Medical Pharmaceutical and Health Sciences, Japan February 2016 until now      
*Sohag Faculty of Medicine, Sohag University, Egypt. September, 2013 until now.

Subject of doctorate thesis: Establishing Hematopoietic Stem Cell Lacking A Human Leukocyte Antigens Haplotype from Induced Pluripotent Stem Cells Derived from Patients with Aplastic Anemia


M.Sc:“Internal Medicine”: Sohag Faculty of Medicine, Sohag University, Egypt. December, 2012 


M.B.B.Ch.: Sohag Faculty of Medicine, Sohag University, Egypt. September, 2007.


General grade: Excellent with Honors


Interest: Hematology. Induced Pluripotent Stem Cell, immune pathophysiology Aplastic anemia, Hematopoietic Stem Cell Transplantation, leukemia, lymphoma, Cancer immunotherapy.

I am very interested in studing Induced Pluripotent Stem Cell, immune pathophysiology Aplastic anemia, Hematopoietic Stem Cell Transplantation, leukemia, lymphoma, Cancer immunotherapy and the immunopathophysilogy and maliganate evolution of hematological diseases using the iPSC technology

I studied the HLA allelic loss frequencies of AA patients with HLA-class I alleles lacking as a result of copy number neutral loss of heterozygosity of the short arm of chromosome 6 (6pLOH) from total 618 AA patients. Then, I cultured iPSCs with different HLA genotypes from monocytes of patients with acquired aplastic anemia (AA) possessing HLA-lacking leukocytes. Second, he induced HSC differentiation from these iPSC clones using different culture systems. Next, I investigated the genotype and the phenotype of iPSC-derived HSPCs (iCD34+ cells) with regard to cell surface markers and type of mutations, then studied the correlation of the different genotype and phenotype of iCD34+ cells with their proliferation and clonogenic potential.

These novel iCD34+ cells allowed to investigate the in vivo behavior and clonogenicity of AA iPSC-derived wild type (WT) and HLA(-) HSPCs without the influence of autologous mature T cells.  For this study, genetically modified immunodeficient mice (BRGS mice) were available and reconstituted with HSPCs derived from two AA patients which included wild-type, 6pLOH(+), and HLA-B allele(-) (B4002[-] or B5401[-]) iCD34+ cells, negative control mice were included. The engraftment of human WT and HLA(-) iCD34+ cells at weeks 9, 10, and 12 after transplantation were extensively investigated by flow cytometry, PCR analysis and immunohistochemistry for determination the multilineage reconstitution of myeloid cells (CD33+), B cells (CD19+), and T cells (CD3+) in the bone marow, spleen, peripheral blood, and lymphoid cells in the thymus with no significant difference in the human-to-mouse chimerism ratio among the 3 groups.

I investigated with the other staff members of the hematology department, Kanazawa university and Fujita Health University the long standing question whether or not patient-derived cytotoxic T lymphocytes (CTLs) would be capable of killing autologous HSPCs using these iCD34+ cells with different HLA genotypes, and more importantly, if they would be able to discriminate between WT and HLA(-) iCD34+ cells in co-culture experiments. For this study, CTL lines were established from the same patient and cultured with iCD34+ cells, then we analyzed the T cell receptor (TCR) β repertoire of the CTL line capable of preferentially killing WT iCD34+ cells as well as T cells in the bone marrow of the patient obtained before immunosuppressive therapy. Stimulation of the patient’s CD8+ T cells with the WT iCD34+ cells generated a CTL line capable of killing WT iCD34+ cells but not killing B4002(-) iCD34+ cells. These data suggest that B4002(-) iCD34+ cells show a repopulating ability similar to WT iCD34+ cells when autologous T cells are absent, and CTL precursors capable of selectively killing WT HSCs are present in the patient’s peripheral blood

 

(III)TEACHING EXPERIENCES:
❖ Undergraduate Internal Medicine course in Faculty of Medicine and Nursing, Egypt .
❖ Undergraduate clinical course of Internal Medicine in Faculty of Medicin, Egypt.
❖ House officer clinical course of Internal Medicine in Faculty of Medicin, Egypt.
❖ Sharing in teaching of Postgraduate Internal Medicine course of MSc students, Egypt.
❖ Sharing in teaching of Postgraduate Stem cells, induced pluripotent stem cells (iPSCs) culturing and differentiation course Cellular Transplantation Biology (Hematology) Faculty of Medicine, Kanazawa University, Japan.


(IV) PROFESSIONAL SCIENTIFIC AND ACADEMIC MEMBERSHIP
❖ Member of Egyptian Stem Cell Transplantation & Hematologic Disease Association
❖ Member of Stem cells reseraches center in Sohag University.
❖ Reviewer in many International Journals.


(V)EXPERIMENTAL WORKS: Extended over the two years in Japan and they included:
• I have skilled in culturing induced pluripotent stem cells (iPSCs) with different HLA
genotypes from the monocytes of patients with aplastic anemia possessing HLAlacking leukocytes, and achieved induction of hematopiotisc stem progenitor cell
(HSPC) differentiation from these iPSC clones using different culture systems.
• Through these experiments, I have performed and skilled
❖ Flow cytometry,
❖ Different types of PCR,
❖ Immunohistochemistry & Immunoflorcense analysis,
❖ Magnetic and fluorescent activated cell sorting (MACS &FACS),
❖ Transfection & Transduction and Cloning,
❖ Clonogenic assay of hematopoietic progenitors
❖ Western blotting analysis.
❖ Enzyme-linked immunosorbent assay (ELISA).
❖ Genetic mutations analysis.
❖ Handling of experimental animals.
❖ Creation of animal model of hematological malignancy for drugs studies
❖ Adminstration of drugs to experimental animals by various routes.
❖ Preparation of cells for examination by different types of microscopes.
❖ Using radioactive chromium (Cr) release assay using a gamma counter.
• I used genetically modified immunodeficient mice (BRGS mice) to reconstitute them with
HSPCs derived from iPSCs after intra-femur injection.

• I have skilled in collection and analysis of different mice organs (bone marrow, peripheral
blood, spleen, and thymus) and determination of the engrafment of human cells.
• I have also skilled in the assessment of cytotoxicity by T cells using 51Cr release assay,
measuring the release of levels of IFN-γ in the cultured supernatants that are determined
by an enzyme-linked immunosorbent assay (ELISA), using a CD107 degranulation
assay with flow cytometry, and counting dead cells using an inverted microscope.


(VI)EXPERIENCES AND SCIENTIFIC ACTIVITIES:
➢ Clinical skills for evaluation of patients with various hematological diseases, and
acquired updated knowledge on the most recent investigations and tretments.
➢ Experince in stem cell transplant and procedures of cord blood transplantation,
peripheral blood stem cells transplantation and bone marrow transplantation for patients
with hematological malignancies or bone marrow failure syndromes.
➢ Good experience in diagnosis &treatment of Internal Medicine diseases.
➢ Practiced and acquired skills in management and dealing with internal medicine
emergency states.
➢ Good experience in dealing with Plasma exchange.
➢ Being as lecturer in the Internal Medicine department;
▪ I am sharing in clinica teaching of undergraduate students
▪ I am sharing as well in training, house-officers and residents in the department.
➢ Attending some of the conferences in the field of Hematology in Egypt and Japan.
➢ Attending some of the conferences in the field of Internal Medicine in Egypt.
➢ Attending some of the workshops and EACCME in the field of Hematology.
➢ Attending and sharing in the weekly “Journal club” and “the research progress
meeting conference” of Cellular Transplantation Biology (Hematology) department
throughout my two year stay in Kanazawa.


(VII) ORAL PERESENTATIONS
• The hematopoietic reconstitution of mice with HLA(-) HSPCs derived from aplastic anemia patients. (79th Annual Meeting of the Japanese Society of Hematology, 22 October 2017, Tokyo, Japan)
• The Promise and Challenge of Induced Pluripotent Stem Cells (iPSCs) in hematology. (Clinical pathology department conference, Faculty of medicine, Sohag University, 16 March 2018, Egypt)

(VIII) POSTER PERESENTATIONS
• Escape Hematopoiesis By HLA-B5401-Lacking Hematopoietic Stem Progenitor Cells in Male Patients with Acquired Aplastic Anemia. (60th American Society of Hematology Annual Meeting and Exposition, December 1-4, 2018, San Diego, USA)
• Identification of T cell receptors specific to antigens presented by HLA-B5401 on IPS cell-derived hematopoietic stem cells in a patient with Acquired Aplastic Anemia Carrying B5401-Lacking Leukocytes . (59th American Society of Hematology Annual Meeting and Exposition, December 9-12, 2017, Atlanta, USA)


(IX) PUBLICATION ACTIVITIES
1. Escape hematopoiesis by HLA-B5401-lacking hematopoietic stem progenitor cells in men with acquired aplastic anemia. Mahmoud I. Elbadry, Hiroki Mizumaki, Kohei Hosokawa, J. Luis Espinoza, …….,Haematologica. 2019 Oct; 104(10): e447–e450.

2. Clonal hematopoiesis by SLIT1-mutated hematopoietic stem cells due to a breakdown of the autocrine loop involving Slit1 in acquired aplastic anemia. Kohei Hosokawa, Hiroki
Mizumaki, Mahmoud I Elbadry, Chizuru Saito, J Luis Espinoza, ……Leukemia.2019 June; 33,2732–2766.

3. Disease modeling of bone marrow failure syndromes using iPSC-derived hematopoietic stem progenitor cells. Mahmoud I Elbadry, J Luis Espinoza, Shinji Nakao.
Experimental hematology. 2019 March;71: 32-42

4. Hematopoiesis by iPSC-derived hematopoietic stem cells of aplastic anemia that escape cytotoxic T-cell attack. JL Espinoza, MI Elbadry, K Chonabayashi, Y Yoshida, T Katagiri, ...Blood advances 2 (4), 390-400 (2018).

5. Escape Hematopoiesis By HLA-B5401-Lacking Hematopoietic Stem Progenitor Cells in Male Patients with Acquired Aplastic Anemia. Kohei Hosokawa, Mahmoud Ibrahim
Elbadry, Hiroki Mizumaki, Luis Espinoza, Noriharu Nakagawa, Kazuhisa Chonabay….. Blood, 2018 November; 132: 3855-3855

6. Identification of T Cell Receptors Specific to Antigens Presented By HLA-B5401 on IPS Cell-Derived Hematopoietic Stem Cells in a Patient with Acquired Aplastic Anemia
Carrying B5401-Lacking Leukocytes. N Nakagawa, MI Elbadry, Y Akatsuka, H Hamana, K Shitaoka, Y Yoshida, ...Blood 130 (Suppl 1), 2458-2458 (2017).

7. The hematopoietic reconstitution of mice with HLA(-) HSPCs derived from aplastic anemia patients. MI Elbadry, JL Espinoza, K Chonabayashi, Y Yoshida, T Katagiri,
...79th Annual Meeting of the Japanese Society of Hematology 22 October (2017)

8. Protective effects of KIR-2DS5, KIR-2DL5 on EBV-related iatrogenic lymphoma (IALPD). NTM Anh, T Imi, MI Elbadry, S Nakao.79th Annual Meeting of the Japanese
Society of Hematology 22 October (2017)

9. The simultaneous inhibition of the mTOR and MAPK pathways with Gnetin-C induces apoptosis in acute myeloid leukemia. JL Espinoza, MI Elbadry, M Taniwaki, K Harada,
LQ Trung, N Nakagawa, ...Cancer letters 400, 127-136 (2017)

10.Induced pluripotent stem cell technology: A window for studying the pathogenesis of acquired aplastic anemia and possible applications. MI Elbadry, JL Espinoza, S Nakao.
Experimental hematology 49, 9-18 (2017)

11.After Moving of Regulatory T-Cell Therapy to the Clinic: Will We Need a New Tregs Source. MI Elbadry, AKA Noreldin, HA Hassanein. Hematol Transfus Int J 5 (2),
(2017)

12.A functional polymorphism in the NKG2D gene modulates NK-cell cytotoxicity and is associated with susceptibility to human papilloma virus-related cancers. JL Espinoza, VH
Nguyen, H Ichimura, TTT Pham, MI Elbadry, CH Nguyen, TV Pham, ...Scientific reports 6, 39231 (2016)

13.An altered gut microbiota may trigger autoimmune-mediated acquired bone marrow failure syndromes. JL Espinoza, MI Elbadry, S Nakao. Clinical immunology (Orlando,Fla.) (2016)

14.Association between rs1761667 polymorphism of CD36 gene and risk of coronary atherosclerosis in Egyptian population. A Boghdady, UA Arafa, EA Sabet, E Salama, A El Sharawy, MI Elbadry. Cardiovascular diagnosis and therapy 6 (2), 120 (2016)

15.Selective immunoglobulin M deficiency in an adult with miliary tuberculosis: A clinically interesting coexistence. A case report and review of the literature. HA Hassanein, MI
Elbadry. International journal of mycobacteriology 5 (1), 106-110 (2016)

16.Study of patients with nephrotic syndrome in Sohag University Hospital. ATA Hassan, AKA Noreldin, MIE Badry. The Egyptian Society of Nephrology and Transplantation
16 (1), 21-31 (2016).

17.Comparison of Successful Myocardial Reperfusion and Adverse Events in Patients With ST-Elevation Myocardial Infarction Who Underwent Rescue Percutaneous Coronary
Intervention After Failed Fibrinolytic Therapy With Versus Without Manual Coronary Thrombus Aspiration. A Boghdady, MI Elbadry. The American journal of cardiology
116 (8), 1185-1192 (2015)

18.A case report of Thrombotic Thrombocytopenic Purpura Associated with Systemic Lupus Erythematosus: Overlapping Features. ATA Hassan, MI Elbadry, M Adel. American
Journal of Medical Case Reports 2,(10), 206-213 (2014).

19.Epidermodysplasia Verruciformis Associated with Astrocytoma, Mantle Lymphoma and Hepatitis B Virus Infection. MI Elbadry, A Othman. American Journal of Medical Case
Reports 2 (9), 187-193 (2014)

 

(X) ONLINE ADDRESS:


Google Scholar URL: https://scholar.google.com/citations?view=&use=&user=CxkTpWIAAAAJ

Linked in URL:https://www.linkedin.com/in/mahmoud-i-elbadry-4b51a0a3/

ResearchGate :https://www.researchgate.net/profile/Mahmoud_I_Elbadry

ORCID iD:

 

E-mail: mahmoudibrahim@med.sohag.edu.eg, mahmoudibrahem837@gmail.com mahmoudibrahem83@yahoo.com            


2018-09-22 13:10:27
The hematopoietic reconstitution of mice with HLA(-) HSPCs derived from aplastic anemia patients
79th Annual Meeting of the Japanese Society of Hematology being held in Tokyo Read more

2018-09-22 13:04:46
•	The Promise and Challenge of Induced Pluripotent Stem Cells (iPSCs) in hematology
Clinical pathology department conference, Faculty of medicine, Sohag University Read more

2020-12-09 15:05:30 | Keywords Hematohidrosis,
Hematohidrosis: Reports and update of clinically mysterious phenomenon
Hematohidrosis is a mysterious and rare disorder characterized by one or more attacks of spontaneous, bloody sweating from intact surfaces of skin and/or mucous membranes. In the current literature, we faced a case of a 16-year old female who presented with recurrent attacks of right-sided bloody otorrhea upon exposure to extreme stress and anxiety. The patient had no history of ... Read more

2020-12-09 15:02:11 | Keywords Mutations Aplastic Anemia,
A frequent nonsense mutation in exon 1 across certain HLA-A and -B alleles in leukocytes of patients with acquired aplastic anemia
Leukocytes that lack HLA allelic expression are frequently detected in patients with acquired aplastic anemia (AA) who respond to immunosuppressive therapy (IST), although the exact mechanisms underlying the HLA loss and HLA allele repertoire likely to acquire loss-of-function mutations are unknown. We identified a common nonsense mutation at position 19 (c. 19C> T, p. R7X) in exon 1 (Exon1 mut) ... Read more

2020-12-09 14:59:02 | Keywords NK Cells Aplastic Anemia,
Resistance of KIR Ligand–Missing Leukocytes to NK Cells In Vivo in Patients with Acquired Aplastic Anemia
The loss of killer cell Ig-like receptor ligands (KIR-Ls) due to the copy number–neutral loss of heterozygosity of chromosome 6p (6pLOH) in leukocytes of patients with acquired aplastic anemia (AA) may alter the susceptibility of the affected leukocytes to NK cell killing in vivo. We studied 408 AA patients, including 261 who were heterozygous for KIR-Ls, namely C1/C2 or Bw6/Bw4, ... Read more

2020-12-09 14:37:19 | Keywords sickle cell,
THE HETEROZYGOUS S HEMOGLOBIN VARIANT DISORDER:  PRACTICAL CONSIDERATIONS
Background: The S haemoglobin variant or sickle cell (SC) disorder is an inherited condition; a person may have sickle cell disease (SCD) or trait (SCT) if he/she inherited two copies of the mutated gene or single copy (heterozygous), respectively. SCT is commonly a neglected hematologic disorder with accidental discovery. Detection of SCT subjects is vital to prevent incidence of serious ... Read more

2020-02-26 23:47:29 | Keywords Acquired aplastic anemia, HLA-B5401-lacking, Hematopoiesis,
Escape hematopoiesis by HLA-B5401-lacking hematopoietic stem progenitor cells in men with acquired aplastic anemia
Leukocytes that lack HLA class I alleles derived from hematopoietic stem and progenitor cells (HSPC) that undergo copy number-neutral loss of heterozygosity of the short arm of chromosome 6 (6pLOH) or HLA allelic mutations are often detected in patients with aplastic anemia (AA). The presence of HLA class I allele-lacking leukocytes provides compelling evidence that cytotoxic T-lymphocytes (CTL) are involved ... Read more