_C. PERFRINGENS_ ALPHA TOXIN : _C. PERFRINGENS_ ALPHA TOXIN : -LECITHINASE (PHOSPHOLIPASE C) -LYSES ERYTHROCYTES, PLATELETS, LEUCOCYTES, ENDOTHELIAL CELLS -MASSIVE HEMOLYSIS AND TISSUE DESTRUCTION إقراء المزيد

NAGLERS TEST : IS IMPORTANT FOR THE IDENTIFICATION OF ALPHA TOXIN OF _CLOSTRIDIUM PERFRINGENS_, THE ADDITION OF ANTITOXIN TO ONE HALF OF EGG YOLK AGAR PREVENTS VISIBLE OPACITY, DUE TO LECITHINASE ACTION WHICH IS NORMALLY OBSERVED AROUND COLONIES. OTHER SPECIES OF _CLOSTRIDIUM_ SHOWS NEGATIVE NAGLERS REACTION_NAGLERS ... إقراء المزيد

Bacterial Neuraminidase "Sialidase" : Function: hydrolyses serum glycoproteins Source: 1-_Clostridium perfringens _ 2-_Vibrio cholerae_ إقراء المزيد

_C. DIFFICILE_: _C. DIFFICILE_: -GRAM + ANAEROBIC ROD. -FOUND IN NORMAL FLORA -OPPORTUNISTIC PATHOGEN إقراء المزيد

_C. DIFFICILE_ TOXIN : 1-TOXIN A : ENTEROTOXIN HYPERSECRETION OF FLUID 2-TOXIN B :CYTOTOXIN CYTOPATHIC TO TISSUE MONOLAYERS_C. DIFFICILE_ TOXIN : 1-TOXIN A : ENTEROTOXIN HYPERSECRETION OF FLUID 2-TOXIN B :CYTOTOXIN CYTOPATHIC TO TISSUE MONOLAYERS إقراء المزيد

_C. DIFFICILE_ Cytotoxin Assay: =Slurry of stool centrifuged =Filter through 0.45 u filter =0.1ml - supernatant to buffer at pH 7.2 =WI-38 tissue cells human diploid lung fibroblasts =Add supernatant to tissue culture. =Observe for cytotoxicity 24 hrs. إقراء المزيد

PMC (PSEUDOMEMBRANOUS COLITIS): REFERS TO SWELLING OR INFLAMMATION OF THE LARGE INTESTINE (COLON) DUE TO AN OVERGROWTH OF CLOSTRIDIUM DIFFICILE (C DIFFICILE) BACTERIA. THIS INFECTION IS A COMMON CAUSE OF DIARRHEA AFTER ANTIBIOTIC USE إقراء المزيد

EPSILON TOXIN OF CLOSTRIDIUM PERFRINGENS TYPE B AND D: =PRODUCED AS AN INACTIVE PROTOXIN =ACTIVATED BY TRYPSIN =INCREASES INTESTINAL PERMEABILITY =INCREASES VASCULAR PERMEABILITY =VASCULAR DAMAGE AND EDEMA IN BRAIN, HEART, LUNG AND KIDNEYS =USED IN BIOLOGICAL WEAPONS إقراء المزيد

GOLDEN STAPHYLOCOCCUS ON BLOOD AGAR إقراء المزيد

_SACCHAROMYCES CEREVISIAE_ COLONIES OF SACCHAROMYCES GROW RAPIDLY AND MATURE IN THREE DAYS. THEY ARE FLAT, SMOOTH, MOIST, GLISTENING OR DULL, AND CREAM IN COLOR. THE INABILITY TO USE NITRATE AND ABILITY TO FERMENT VARIOUS CARBOHYDRATES ARE TYPICAL CHARACTERISTICS OF SACCHAROMYCES._SACCHAROMYCES CEREVISIAE_ COLONIES OF SACCHAROMYCES GROW RAPIDLY ... إقراء المزيد