Differentiation Study Between Alfalfa Mosaic Virus and Red Clover Mottle Virus Affecting Broad Bean by Biological and Molecular Characterization
This study aimed to identifying the causal virus (es) inducing wilting and necrotic symptoms on broad bean plants. Amplification of total RNA extracted from infected broad bean yielded 200-550 bp using Comovirus-specific primers. The amplified cDNAs was cloned, sequenced and analyzed. Nucleic acid sequence analysis of smallest and biggest bands revealed of 100 and 95% sequence identity with other Red ... إقراء المزيد