Mould occurrence and growth in poultry farms is one of the major threats to poultry economy and health. The aim of this study was to investigate the mycological contamination of poultry feeds used in poultry farms in Sohag Governorate, Egypt. A total of 160 samples of poultry feed were collected from 10 farms for mycological analysis. The isolated fungi from these samples were cultured in Sabouraud Dextrose Agar and Potato Dextrose Agar at 25oC and 37oC for two weeks. Positive cultures were identified through standard methods. The mycological examination revealed the isolation of 8 fungal species and 102 (63.7%) of poultry farms in the locality had mycotic contamination. The most prevalent fungi species were Fusarium solani (24.5%) and Aspergillus flavus (17.65%), followed by Aspergillus niger (14.71%), Penicillium chrysogenum (11.76%), Mucor species (9.8%), Rhizopus species (8.82%), Cladosporium species (7.84%) and the least frequency species were from genus Alternaria species (3.92%). These findings therefore suggest that poultry feed provides a good ecology for fungi thereby posing a great public health threat to humans.