Complexes of Fe (II) and Cu (II) with a tridentate Schiff base, 2-((z)-(pyridine-2-ylimino) methyl) naphthalene1-ol derived from 2-hydroxy-1-napthaldehyde and 2-aminopyridine were synthesized. Both the ligand and its complexes were characterized on the basis of microanalysis, melting point, 1H and 13CNMR, molar conductivity, thermal analysis, IR and UV/Vis spectral studies. From analytical data, the stoichiometry of the complexes was found to be 1: 2 (metal: ligand). The magnetic susceptibilities of all complexes at room temperature were consistent with octahedral geometry. The molar conductance values suggest nonelectrolytes. The IR spectra revealed that the metal ions coordinated through azomethine nitrogen, pyridine nitrogen of and phenolic oxygen of the ligand. The particle size of iron and copper complexes has been investigated by TEM. Antibacterial and antifungal activities in vitro were performed against three types of G+ and Gbacteria, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and three types of fungi, Aspergillus flavus, Trichophyton rubrum and Candida albicans with determination of minimum inhibitory concentrations of ligand and metal complexes. Both complexes showed highly effective antibacterial activities against the tested bacteria and fungi; therefore, these complexes can be used as antibiotic. Furthermore, DNA interaction of Schiff base complexes was monitored by electronic spectra, hydrodynamic measurements and gel electrophoresis. It was found that the prepared complexes could bind to DNA in an intercalating mode