This study aimed to describe the normal ultrasonographic image of the gastrointestinal tract and spleen in
Egyptian buffaloes (Bubalus bubalis). The obtained results were compared to the normal reference values that
had been previously established in cattle. This study was carried out on thirty healthy buffalo. Complete clinical
examination, hematological pictures profiling, blood biochemical analysis of different parameters such as total
blood serum proteins, albumins, globulins, aspartate aminotransferase, alkaline phosphatase, gamma-glutamyl
transferase, triglycerides and cholesterol, radiography and ultrasonographic examination were done. The clinical
findings, blood pictures and blood biochemical levels agreed with the previously reported reference values of
healthy buffaloes. Radiography of the healthy buffaloes showed free reticulum, normal heart and well-identified
diaphragm. The heart appeared as radio-opaque structure. This study could detect the suitable site for
visualization of reticulum, omasum, abomasums, rumen, small intestine and large intestine. It also described the
normal ultrasonographic patterns of these organs. It was very essential to establish these ultrasonographic
findings to improve the diagnostic efficacy of ultrasonography for evaluation of different gastrointestinal organs
in healthy buffaloes that will be very useful in the future detection of the abnormal findings associated with
gastrointestinal diseases.