Moringa oleifera is a fast growing tree under field conditions. A micropropagation
protocol of M. oleifera was established, and effects of salinity and ventilation on fast growing
cultured plant materials were studied. Efficient shoot formation and multiplication were obtained
when nodal or shoot tip explants were cultured on MS medium supplemented with 2.5 μM
6-benzylaminopurine (BAP) at mild ventilation conditions. Adventitious root formation was
obtained when shoot cuttings were cultured on MS medium supplemented with 4.92 μM indole-
3-butyric acid (IBA). Under salinity, M. oleifera shoots showed symptoms of vitrification such
as retardation in shoot multiplication and growth, stimulated callus formation on the basal part of
the cultured explants and increased shoot thickness. M. oleifera shoot multiplication and growth
were negatively affected by ventilation deficiency.