Trichomonas vaginalis, a flagellate protozoan parasite inhabiting the urogenital tract of humans and it is the most common nonviral sexually transmitted pathogen. This is a cross section study done upon 43 ladies complaining of leucorrhea who attended the outpatient gynecology clinic of Sohag University hospitals during the period between January 1st and April 30th,2011. High vaginal sterile swabs were taken to be used for wet mount preparation, Giemsa stain and culture on TYM medium for detection of T. vaginalis . Both Giemsa stain and wet mount preparation could detect the protozoan in 5 out of the 43 patients (11.6%), while the culture yielded positive results in 12 cases (27.9%), the difference was highly statistically significant( p value of 0.0046). all cases who were positive at wet mount and Giemsa stain preparation were positive at culture, whereas of the 38 women who gave negative results at wet mount and Giemsa stain, seven women were positive at culture. This means that both wet mount  and Giemsa stain are poorly sensitive to detect culture positive cases (41.6%), but highly specific (100%); i.e. a good positive test and resorting to culture will give a significantly better chance for the diagnosis.