Introduction:   Acrosome  has a vital role in the process of penetration of zona pellucida (ZP). It is highly susceptible to elevated reactive oxygen species (ROS) and oxidative stress (OS). As generally accepted that OS stress has deteriorating effects on sperm functions,  we studied the effect of  OS  on  sperm  parameters and  acrosomal   structure   in  infertile  patients  with  normal  semen parameters.

Patients  and methods:  30 infertile patients  with normal  semen parameters and 20 normal  fertile controls were included in this study. Semen analysis was performed  according to WHO parameters. Oxidative stress was evaluated by measurement  of Malondialdehyde (MDA). Acrosomal  anomalies were detected  by transmission electron microscopy  (TEM).

Results:   Statistically  no significant difference was found  between infertile patients  and controls regarding  semen parameters (p value >0.05). MDA  values were statistically  highly significant  in infertile  patients  than  normal  controls  (p value <0.001). Acrosomal  anomalies  were statistically highly significant in infertile patients  than normal  controls  (p value <0.001). Acrosomal  anomalies were positively correlated  with MDA values. Pearson correlation was 0.645 for correlation between redundant or detached  acrosome  and  MDA  values and  0.707 for correlation between acrosomal inclusions and MDA  values. Conclusion:  Percentages  of  MDA   values  and  acrosomal   anomalies   were  higher  in  infertile patients  than  normal  subjects.  The positive correlation between acrosomal  anomalies  and  MDA values means association  between OS and acrosomal  anomalies which may indicate negative effects of OS on the acrosomal  structure.