Surrogate Role of CD85k on Monocytic Lineage Involved Leukemogenesis Biology and Clinical Aspect

Surrogate Role of CD85k on Monocytic Lineage Involved Leukemogenesis Biology and Clinical AspectHasnaa A Abo-Elwafa1, Heba A E Ahmed1, Elsayed M Ali2, Doaa S Elsaied1, and Azyzi P S 1Clinical Pathology Department1and Clinical Oncology Department, Sohag Faculty of Medicine, Sohag University, EgyptCorresponding author: Hasnaa Ahmed Abo-ElwafaAssistant Prof. of Clinical Pathology, Faculty of Medicine, Sohag University, EgyptHasnaa_aboalwafa@med.sohag.edu.eg    Open Journal of Blood Disease, 2017 http://www.scirp.org/journal/ti  ISSN Online: 2164-3199

Abstract

Abstract, background, unique receptor involved in leukemogenesis is CD85k; an immunoglobulin receptor for immune tolerance, CD36 is glycoprotein mediates cellular adhesion and metastatic spread, CD14,CD15 considered common monocytic markers, aims to investigate CD85k with monocytic lineage involved leukemia (MLIL) markers in leukemia pathogenesis and clinical presentation, patients and methods, 47 patients (32 diagnosed acute myeloid leukemia( AML); 15 non-malignant hematological disease as a control), were included, aged from 2 to 80 years, all subjected to peripheral blood (P.Bl) and bone marrow (B.M) examination, immunophenotyping (IPT) using FASC Canto four color flow cytometer (FCM) Becton Dickenson(BD) USA, for CD13,CD33, MPO, HLA-DR, CD34, CD38, CD117,CD14, CD15 and CD36 the Mo Abs supplied by B.D Bioscience,  and anti CD85k Mo Abs by Aveda de Coimbra Flamenco, reference No. 1399990130, results, frequency of CD85k is 19/32 ( 59.37%) of AML; 14/14 (M4/M5) 100% positive CD85k, insignificant correlations of CD85k to sex, lymphadenopathy or organomegaly, platelets count and P.Bl blast (P> 0.05), significant to age < 35years, WBCs > 50,000x10⁹/l , Hb < 7 g/dl, BM blasts, CD34 and HLA-DR CD33, CD13, CD38 (P < 0.05), insignificant correlations to CD36, CD14, CD15and CD117( P> 0.05), conclusion, although CD85k is MLIL associated marker it is not correlated with other MLIL markers, has frequency 100% in MLIL and 59.37% in AML, age predisposition is < 35years with no sex variation, significant correlation to progenitor and myeloid markers, its crucial role in leukemogenesis biology , not in clinical presentations, considered good follow up predictor MLIL marker.

Keywords

CD85k, Monocytic Lineage Leukemia