association of between two common mutations C677T and A1298T in human Methylene tetrahydrofolate reductase enzyme gene and the risk for diabetic nephropathy in type II diabetic patients of

Diabetic nephropathy(DN) is a serious microvascular complication of diabetes and is the leading cause of end stage renal failure .Mutations of methyelene tetrahydrofolate reductase (MTHFR) gene have been shown to be associated with a predisposition to DN in some populations.This study aimed to investigate whether polymorphisms of(MTHFR)C677T(rs 1801133) and A1298C(rs1801131)considered arisk factor for DN among type2 diabetes mellitus patiens(T2DM) or not.This study conducted to 135 individuals,75 T2DM patients and 60 age and sex matched healthy volunteers.The (rs1801133) and (rs1801131)polymorphisms were assessed using polymerase chain reaction (PCR)and restriction fragment polymorphism(RFLP)analysis.

The presence of (rs1801133) mutant genotype increased the risk of DN 8.5 times(OR=8.5,95%CI=2.25-22.48,p=0.0001).The presence of T allele increased the risk 4.8 times(OR=4.89, 95%CI=2.25-10.66,P=0.0001).The presnence of (rs1801131)mutan genotype or the mutant C allelewas not affect the risk of DN

Conclusion:(rs1801133)was a risk factor for DN in T2DM Egyptian patients while (rs1801131)was not.the presenence of these polymorphisms of MTHFR synergistically act to increase risk of DN.