Brucellosis is one of the most important diseases affecting both human and animal in most of the developing countries.  It causes important economic losses. Rapid and accurate diagnosis is fundamental for control and eradication of brucellosis.  

A total of 150 milk samples (three samples from each animal included in the study) and 50 blood samples were collected from 32 cows and 18 buffaloes suspected to be infected or had a history of brucellosis from villages and cities of Sohag, Beni-Suef and Giza governorates in Egypt. All tested animals gave a negative reactions to all serological test that included Buffered acidified plate antigen test (BAPAT), Rose bengal plate test (RBPT), Tube agglutination test (TAT) and Rivanol test (Riv.T).

Bacteriological  and molecular  examination  of  milk  samples  revealed  detection  of  Brucella  organisms  in 40.6% and  53.1%  from  the  cow's  samples  by  the  two  examinations  respectively  and  in  38.9% and  55.6% from the buffaloe's samples by the two examinations respectively. All the isolated brucella organisms were B. melitensis.

Out of 32 cow′s serum samples examined by BAPAT, RBPT, TAT and Riv.T, antibodies against Brucella were found in 32 samples (100%), 30 samples (93.8%), 29 samples (90.6%) and 27 samples (84.3%) by these tests respectively. Out of 32 cow ′s milk samples examined by MRT, antibodies against Brucella were found in 28 samples (87.5%). On the other hand, Out of 32 whey samples examined by wBAPAT, wRBPT, wTAT and wRiv.T, antibodies against Brucella were found in 25 samples (78.1%), 24 samples (75.0%), 24 samples (75.0%) and 23 samples (71.9%) by these tests respectively.  

Out of 18 buffalo ′s serum samples examined by BAPAT, RBPT, TAT and Riv.T, antibodies against Brucella were  found  in  18  samples  (100%)  by BAPAT  and  in  16  samples  (88.9%)  by  each  from RBPT, TAT  and Riv.T. Out of 18 buffalo′s milk samples examined by MRT, antibodies against Brucella were found in 15 samples (83.3%). On the other hand, Out of 18 whey samples examined by wBAPAT, wRBPT, wTAT and wRiv.T, antibodies against Brucella were found in 14 samples (77.8%) by each of them.