Ten okra (Abelmoschus esculentus (L) Moench.) genotypes were evaluated for their agronomic traits and molecular diversity. Four agronomic traits, i.e. number of days to 50% flowering; plant height; number of pods per plant and total yield per plant were evaluated. Amplification of genomic DNA of the ten genotypes using ISSRs. Seven primers generated 40 fragments, of which 15 were polymorphic ... إقراء المزيد

Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene NOT LIKE ... إقراء المزيد

Genetic variability and identification of some molecular markers were studied in twenty promising lines of wheat using agronomic traits, ISSR (inter simple sequences repeats) and RAPD (random amplified polymorphic DNA) markers. Significant variation was evidenced in all agronomic traits. The lines proved to be superior to the check cultivar Sahel1 in yield and its component traits. Lines L2, L7 and ... إقراء المزيد

Banana plants showing typical mosaic and yellow stripes on leaves as symptoms were collected from Assiut Governorate in Egypt. The causal agent was identified as Cucumber mosaic virus (CMV) on the basis of symptoms, transmission, serology, transmission electron microscopy (TEM) and reverse transcription polymerase chain reaction (RT-PCR). Coat protein (CP) gene was amplified using gene specific primer, followed by cloning ... إقراء المزيد

Maize endosperm is both a crucial contributor to world nutrition and a model system for plant developmental biology. For both aspects promoters with expression zones restricted to particular domains of the endosperm would be a valuable tool. The ˇ-glucuronidase (Gus) reporter gene was fused to upstream fragments of three genes whose expression is limited to specific domains of the endosperm: ... إقراء المزيد

Cucumber mosaic virus (CMV, cucumovirus) is the most important virus infecting cucurbit crops in Egypt and worldwide causing significant loss in yield quality and quantity. The main target of the present work was to establish a simple controlling system for an Egyptian isolate of such virus (belonging to the subgroup I) via production of tobacco transgenic plants expressing viral coat ... إقراء المزيد

ZmOCL1 is the founding member of the ZmOCL (Outer Cell Layer) family encoding putative transcription factors of the HD-ZIP IV class. It is expressed in the L1 cell layer of the embryo and several other tissues of maize. After determination of the intron/exon structure a mutator insertion was isolated in the upstream region. No notable phenotypes and wildtype levels of ... إقراء المزيد

Two novel members of the receptor-like protein kinase (RLK) family were isolated from maize embryos by homology to ZmPK1, the first RLK identified in the plant kingdom. PK8-a and PK8-b share 88% sequence identity and code for deduced proteins with molecular masses of 57.3 and 56.6 K, respectively. The proteins contain a trans-membrane domain close to the N-terminus and a ... إقراء المزيد