Conclusions: The results of this study indicated a significant incresae of SCSA-defined sperm nuclear DNA damage can be found in sperm from infertile men with normal standard semen parameters. Therefore, sperm DNA damage analysis may reveal a hidden abnormality of sperm DNA in infertile men who were previously classified as idiopathic based on apparantly normal standard semen parameters. Read more

OBJECTIVE: To examine the effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. DESIGN: Prospective study. SETTING: Infertility clinic at a tertiary care academic institution. PATIENT(S): Ninety-two infertile men with normal female partners. Sixteen fertile donors served as the control group. INTERVENTION(S): Standard semen analysis and assessment of levels ... Read more

OBJECTIVE: To evaluate levels of sperm nuclear DNA damage in infertile men with normal and abnormal standard semen parameters. DESIGN: Prospective study. SETTING: Male infertility clinic. PATIENT(S): Ninety-two men seeking infertility treatment and 16 fertile volunteers. INTERVENTION(S): Standard semen analysis was performed according to the World Health Organization guidelines. MAIN OUTCOME MEASURE(S): Sperm DNA damage was assessed by sperm chromatin ... Read more

OBJECTIVE: To investigate the role of increased seminal leukocytes in enhancing reactive oxygen species (ROS) production by human spermatozoa. DESIGN: A prospective study. SETTING: Male infertility clinic. PATIENT(S): Forty-eight infertile men. INTERVENTION(S): Standard semen analysis. Assessment of sperm nuclear DNA damage by sperm chromatin structure assay. Incubation of spermatozoa from nonleukocytospermic samples with blood neutrophils. MAIN OUTCOME MEASURE(S): Spontaneous and ... Read more