This paper presents an empirical evaluation of the relationship between operational performance and platooning phenomenon in rural two-lane roads in Egypt. Seven performance measures and three platooning variables were defined and calculated for eight study sites using traffic data from roads in Minoufiya governorate, Egypt. Using graphical and statistical analyses, the associations between the performance measures and the platooning variables were examined. The results showed that the follower density performance measure was found to have the strongest correlations to platooning variables. Among the platooning variables investigated, traffic flow in the direction of travel has the highest correlations with performance measures. The study demonstrated that the relationship between follower density and traffic flow is better described by a quadratic form. Finally, threshold values for different levels-of-service were proposed. This might help traffic engineers, in Egypt, to evaluate operational performance using criteria that reflect the local conditions of the area under study.