Fifty bread wheat lines were evaluated for heat tolerance and compared to some local cultivars under three sowing dates (November 25th, December 15th and January 5th). Four agronomic traits were evaluated, i.e. No. of spikes per plant, grain yield per plant, 100-kernel weight and harvest index under normal and stress conditions. Analysis of variance showed highly significant variations among the tested lines and demonstrating that the main effect of sowing dates was due to the late date. Grain yield per plant was the most affected trait by heat followed by 100-kernel weight and No. of spikes per plant, while harvest index showed the lowest reduction due to heat stress. Six lines (L1, L11, L16, L34, L37 and L41) showed heat tolerance based on high performance in grain yield/plant by 29.45, 29.75, 27.75, 27.43, 27.37 and 31.90 g, respectively under late sowing conditions as well as low heat sensitivity index. The sequence related amplified polymorphism (SRAP) was able to differentiate between bulked DNA samples of lines with the highest and lowest performance in agronomic traits under heat stress. SRAP generated 2, 1 and 3 bands specific for lines with high performance of No. of spikes per plant, grain yield per plant and harvest index, respectively as well as it showed 5 and 3 bands specific for lines with low performance of grain yield per plant and harvest index, respectively. These specific bands could serve in wheat genotyping and screening, and might be used as SRAP markers associated with heat tolerance in wheat breeding programs.