Spread and occurrence of sugar beet rhizomania caused by beet necrotic yellow vein Benyvirus in some Governorates in Egypt

Beet necrotic yellow vein Benyvirus (BNYVV) is the important soilborne virus disease in the production areas of sugar beet. In this study, field survey was carried out in sugar beet growing areas belonging to Kafrel-Sheikh, El-Behera, El-Dakahlia, El-Gharbia and El-Minia Governorates. The results of this survey revealed the presence of characteristic symptoms of rhizomania syndrome, ie stunted and constricted roots which developed a proliferation of rootlets. The foliar symptoms appeared as yellowing rounded patches in the field, including general chlorosis, wilting and necrotic vein yellowing. Samples of rhizomania-like symptom roots and its adjacent soil were taken from these areas and tested for the presence of BNYVV using indirect DAS-ELISA with specific polyclonal antibodies to C-terminal 60 amino acids of BNYVV coat protein and bait plants test, respectively. The results confirmed the presence of BNYVV …