A series of Fe(III), Ni(II) and Cu(II) complexes of the type [Fe(L)(H2O)2Cl].2H2O, [Ni(L)(H2O)].2H2O and [Cu(L)(H2O)3].2H2O, where L bi-negatively tridentate Schiff base ligand derived from condensation between anthranilic acid and 2-hydroxy-benzaldhyde, have been synthesized. The proposed structures of the obtained complexes have been established from elemental analyses, UV-Vis., FT-IR, magnetic moment and conductivity measurements. Density Functional Theory (DFT) calculations were established to estimate the electronic structural parameters to validate the 3D geometry of the prepared compounds. The free ligand and its metal complexes have been screened for their in-vitro antibacterial (Escherichia coli (G-) and Bacillus cereus (G+)) and antifungal (aspergillus fumigatus) activities in terms of the minimum inhibitory concentration (MIC). The results indicate that the biological activity increases on complexation.