Date palm pollen (DPP) is a natural product produced from male palm flowers and is used to improve ovulation and fertility in both women and men because it contains amino acids, fatty acids, flavonoids, saponins and sterols. Thus, this experiment investigated the effect of DPP supplementation on the oviposition rate, egg quality, ovarian follicle development, hematological variables and hormonal profile of laying hens. The findings indicated that the egg weight (EW), egg mass, albumen quality and laying rate of laying hens treated with DPP levels were significantly increased compared to those of the hens in the control group. The increased DPP levels significantly (p < 0.05) increased white blood cells (WBC), hemoglobin (Hb) and total antioxidant capacity (TAC), while H/L ratio significantly decreased. The increased DPP level significantly (p < 0.05) increased follicle-stimulating hormone (FSH) and luteinizing hormone (LH) concentration, ovary and oviduct weights compared to those of the control group.