Abstract The two ruminant parasites, Paramphistomum
cervi and Carmyerius gregarius, were collected from freshslaughtered
native cattle at local abattoirs in Sadat district,
Menoufia province and identified morphologically, then molecularly
by sequencing the nucleotides of 18S ribosomal
RNA gene (18S rRNA). The nucleotide sequences of the
two isolates were 456 (P. cervi) and 401 bases for
(C. gregarius). The data were used along with those of several
other helminth species from the GenBank to identify these
two species genetically. The nucleotide sequences were
aligned using multiple sequence alignments of nucleotides
by Clustal W 12.1 V and construct their relationship.
Neighbor-joining analytical method was used showing sister
relationship between C. gregarius from Sadat district and
Gastrodiscoides hominis (EF027096) with relative identity
of (98%) due to the presence of single nucleotides polymorphisms
(SNPs) in the form of indels as nine nucleotides positions.
But when clustering of P. cervi Sadat isolate with
Paramphistomoidea sp. S4 isolate P5 (GU735643), this relationship
shows complete identity (99%) between them. The
homology and diversity was done using Bayesian analyses in
MrBayes v3.1. This work will give a useful guide for other