The aim of the present work was to detect the incidence rate of Sarcocystis spp. from Egyptian Water Buffaloes (Bubalus bubalis) in
Sohag governorate, Egypt. Blood and muscle samples (145 each) were collected from neighborhood abattoirs. S. buffalonis 33.8%,
S. levinei 14.48% and S. fusiformis 0.41% were recognized by microscopic examination and confirmed by indirect
immunofluorescence antibody technique (IFAT). Results showed that 26.9% examined samples were positive by routine abattoir
examination while 52.4% examined samples were positive by microscopic examination, on the other hand; 64.8% samples were
positive by IFAT. We can concluded that, gross examination at the abattoirs is not adequate to present safe meat for human
utilization, and it must be supported with other serological tests like IFAT, although it has a moderate specificity and high sensitivity
to diagnose Sarcocystosis.