Enteric protozoal parasites are important enteropathogens of wild, domestic animals, and humans; and they are responsible for important zoonotic diseases. Currently, the diagnosis of parasitic infections rely on several laboratory methods, as microscopy that plays a prominent role in the identification of these parasites, also a new immunological methods are continually evolving, most of these tests depending on the detection of an antigen in fecal samples or antibody in serum samples, in the present study, fecal samples were collected from calves, lambs, dogs, and cats, aged from 1 day to 30 in the Menoufiya province. All samples were examined directly by floatation centrifugation technique by using zinc sulphate solution(sp. g:1.18), for Giardia spp, and Entamoeba histolytica, and Modified Sheather’s Solution (SG 1.27) for Cryptosporidium spp. oocysts then stain with the Modified Ziehl-Neelsen method, and were preserved with PBS at -20˚C for detection of copro antigen using antigen capture sandwich ELISA, and immunochromatographic assay, and the aim from this study was to evaluate the sensitivity and specificity of immunodiagnosis in relation to microscopy. Results revealed that, by microscopic exam, a three main protozoa were found in all examined animals with different percentage (Cryptosporidium spp. oocysts, Giardia spp, and Entamoeba histolytica). Direct Eliza test was done on Cryptosporidium spp suspected fecal samples, and immunochromatographic assay was done on Giardia spp, and Entamoeba histolytica positive samples by microscopy, then the sensitivity and specificity and statistical analysis were calculated.