In an attempt to find a new class of antimicrobial agents, a series of pyridoxazepine, pyridothiazepine, triazepine, naphthyridine, pyridopyrimidine, and other related products containing pyridine moiety were synthesized via the reaction of 2‐amino‐6‐chloro‐3,5‐dicyanopyridine 1 with nucleophilic chemical reagents. These compounds were screened for cytotoxicity and their antimicrobial activity. Most of products showed inhibitory effect.