On the basis of fifty morphological characters, including vegetative parts, flowers, fruits, seeds, pollen grains, and anatomical structure, a systematic study of 13 taxa belonging to genus Galium (Rubiaceae) from Egypt was conducted by means of numerical analysis. Four branches and clusters were distinguished. Representatives of these groups were clustered together according to characters with high factor loading in the principal coordinates analysis. The results showed congruence between the UPGMA clustering and principal coordinates analysis in suggesting four groups. There was some degree of similarity among the species of sect. Aparine (Kolgyda). The results indicated also that the sect. Leiogalium (G. mollugo) was a separate group, while Aparine (Kolgyda) was the most heterogeneous one.