The current study was carried out to determine the impact of sucking lice (Haematopinus tubercultus.) infestation on dairy buffalo cows productivity. Twenty four dairy buffalo cows were examined for lice infestation at the Experimental Animal Farm, Faculty of Agriculture, Sohag University, El-Kawamel city, Egypt. Animals were divided into two groups according to infection (14 uninfected animals and10 infected animals). Individual milk yield and body weight for all buffalo cows were weekly recorded for 8 weeks (3 weeks before infestation, 2 weeks during treatment and 3 weeks after treatment). Infected animals were treated with Butox 5% EC at concentration of 2 ml/ liter. The obtained results showed that the milk yield and body weight of infected animals were significantly (P<0.05) decreased during infection and treatment stage than the corresponding value before infection (2.73 vs. 3.97 L and 523.8 vs. 535.4 kg, respectively).Also, these findings indicated that the infected animals were loss 31.18 and 2.17% of milk yield and body weight, respectively. So, farmer should pay attention to improve the ability of immune resistance against ectoparasites and implement a preventive program against ectoparasites which would harmful for dairy buffalo cows productivity, welfare and appearance.