Pyridopyrimidine derivatives 2, 5 and 7–9 were furnished by the reaction of compound 1 with a variety of
reagents, namely, formic acid, acetic anhydride, phenyl isothiocyanate, phenyl isocyanate, carbon disulfide,
acetamide and thioacetamide, which in turn were treated with hydrazine to give pyrazolopyridopyrimidine
derivatives 4, 6 and 11–13. The potency of the results as antibacterial agents has been evaluated. Most of
the tested products showed a highly inhibition zone against the two bacterial strains. All compounds have
been characterized based on their IR, 1H-NMR and Mass spectra.