Abstract
Objectives: Several candidate genes are implicated in the pathogenesis of type 2 diabetes mellitus T2DM), one
of which is interleukin (IL-10). In this investigation, we aimed to study the association between IL (-592A/C) gene
polymorphism with its level in T2DM with and without nephropathy.
Methods: IL-10 (-592A/C) gene polymorphism was genotyped using restriction fragment length polymorphism
(RFLP-PCR) technique and IL-10 levels were measured in two groups of T2DM, one group complicated with
nephropathy and the second non-complicated.
Results: Our results revealed no significant differences in IL-10 (−592A/C) genotypes distribution between the
two groups of T2DM patients. IL-10 levels were found significantly elevated in diabetic nephropathy patients
compared to T2DM without nephropathy (P<0.05) and were positively correlated to the degree of albuminuria
(r=0.61, P<0.01). IL-10 levels increased in IL-10- (−592C/C) genotype compared to IL-10-(−592A/A) and IL-10-
(−592A/C) genotypes in diabetic nephropathy patients while such difference was not found in T2DM patients without
nephropathy.
Conclusion: IL-10 (-592A/C) gene polymorphism and IL-10 level play a role in the pathogenesis of nephropathy
in T2DM.