Background
Acne vulgaris is a common dermatological disease frequently found in late childhood and adolescence. Oxidative stress corresponds to an imbalance between the rate of oxidant production and that of their degradation. Excessive rcactix c oxy gen species (ROS) production may explain, at least in part, the progress or inflammation in the pathogenesis or the disease. Objective
"".'"1 assess tissue levels of oxidative stress indices: Malondialdehyde (MDA) and antioxidants as Supcroxide clismutase (SOD) and catalase (C/\T) in a group of patients with acne as compared to a control group. And to determine the relationship of oxidative stress indices with severity of acne.
Patients and Methods
Two punch biopsies (3 mm) were taken from the skin of patients (one from the back represented acne involved area and another from the forearm represented non acne-involved
- in). One skin biopsy was taken from the forearm of healthy controls. Tissue samples were eighed and homogenized using a glass/glass homogenizer under cold phosphate buffer. The mogenate was then centrifuged at 4000 round per minute (rpm) for 15 min, and the
, pernatant was used for determination of tissue SOD, C/\T activities and MD/\ levels.
Results
ere was statistically significant increase in median enzyme levels of SOD and CAT in acne ir . olved skin as compared to non acne involved skin. There was statistically significant mcrease in median levels of SOD and C/\ T and MDA levels in acne involved skin as rorrpared to normal control. There was statistically significant correlation between SOD and l\1DA levels and severity or acne. There was statistically significant positive correlation vween SOD activity in acne involved and non involved skin. There is statistically si=nificant positive correlation between CAT and SOD in acne involved skin. Also there is star srically significant positive correlation between MDA in acne involved and non involved
ir of the same patient. There was statistically significant negative correlation between
MDAin acne involved skin and SOD in acne involved and non involved skin of the same
patient.
Conclusion
Oxidative stress exists in patients with acne vulgaris and may play a role in the aetiopathogenesis and or progression of the disease. The addition of drugs with antioxidativc effects seems to be valuable in the treatment of acne vulgaris.