Tramadol hydrochloride (T), (±)-cis-2-[(dimethylamino)- methyl]-1-(3-methoxyphenyl)cyclohexanol (1) [1-7] is a centrally acting analgesic agent used in the treatment of moderate to severe pain and as an alternative to opiates [8]. The sensitive analysis for drugs of abuse in seized materials or biological fluids has become important for forensic and toxicological laboratories. High performance liquid chromatography (HPLC) with ultraviolet (UV) [9-13] is ... إقراء المزيد