The biological effect of the perfluorocarbon-based artificial oxygen carrier (OxygentÔ) was investigated in tissue- engineered trachea (TET) construction. Media supplemented with and without 10% Oxygent were compared in all assessments. Partial tissue oxygen tension (PtO2) was measured with polarographic microprobes; epithelial me- tabolism was monitored by microdialysis inside the TET epithelium perfused with the medium underneath. Chondrocyte–DegraPolÒ constructs were cultured ...
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